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rage function blocking antibody  (R&D Systems)


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    R&D Systems rage function blocking antibody
    Mesangial cells were grown for 2 days on tissue culture plastic + AGE-BSA or BSA (20 µg/mL) as in Figure 5 legend. 10 µg/mL rat plasma FN and 15 µg/ml <t>RAGE</t> <t>function-blocking</t> antibody were added for the final 24 hours. (A) Phase and fluorescence images were captured as described in Figure 5 legend. Average FN fluorescence intensity was decreased 1.16-fold to 0.86 ± range (n = 2) in the presence of RAGE function-blocking antibody with AGE-BSA compared to AGE-BSA treatment alone. Scale bar is 200 µm. (B) The DOC-insoluble fraction was separated by SDS-PAGE and immunoblotted with IC3 anti-rat FN monoclonal. Relative densitometry values are the mean of two experiments ± range normalized to GAPDH from the DOC soluble fraction. Blot is representative of two independent experiments.
    Rage Function Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rage+function+blocking+antibody/Human%2FMouse%2FRat+RAGE+Antibody/pmc05237628-374-2-7
    Average 94 stars, based on 47 article reviews
    rage function blocking antibody - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly"

    Article Title: Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    doi: 10.1016/j.matbio.2016.07.003

    Mesangial cells were grown for 2 days on tissue culture plastic + AGE-BSA or BSA (20 µg/mL) as in Figure 5 legend. 10 µg/mL rat plasma FN and 15 µg/ml RAGE function-blocking antibody were added for the final 24 hours. (A) Phase and fluorescence images were captured as described in Figure 5 legend. Average FN fluorescence intensity was decreased 1.16-fold to 0.86 ± range (n = 2) in the presence of RAGE function-blocking antibody with AGE-BSA compared to AGE-BSA treatment alone. Scale bar is 200 µm. (B) The DOC-insoluble fraction was separated by SDS-PAGE and immunoblotted with IC3 anti-rat FN monoclonal. Relative densitometry values are the mean of two experiments ± range normalized to GAPDH from the DOC soluble fraction. Blot is representative of two independent experiments.
    Figure Legend Snippet: Mesangial cells were grown for 2 days on tissue culture plastic + AGE-BSA or BSA (20 µg/mL) as in Figure 5 legend. 10 µg/mL rat plasma FN and 15 µg/ml RAGE function-blocking antibody were added for the final 24 hours. (A) Phase and fluorescence images were captured as described in Figure 5 legend. Average FN fluorescence intensity was decreased 1.16-fold to 0.86 ± range (n = 2) in the presence of RAGE function-blocking antibody with AGE-BSA compared to AGE-BSA treatment alone. Scale bar is 200 µm. (B) The DOC-insoluble fraction was separated by SDS-PAGE and immunoblotted with IC3 anti-rat FN monoclonal. Relative densitometry values are the mean of two experiments ± range normalized to GAPDH from the DOC soluble fraction. Blot is representative of two independent experiments.

    Techniques Used: Clinical Proteomics, Blocking Assay, Fluorescence, SDS Page

    Related Articles

    Blocking Assay:

    Article Title: Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly
    Article Snippet: In experiments with exogenous AGE-BSA, 20 μg/ml AGE-BSA (Millipore, Darmstadt, Germany) or fatty acid-free BSA (Sigma, St. Louis, MO, USA) was added to the media at 0 and 24 hours into a 48-hour time-course. .. 15 μg/ml RAGE function blocking antibody (AF1179, R&D Systems, Minneapolis, MN) or lysyl oxidase (LOX) antibody (ab31238, Abcam, Cambridge, MA) was added for final 24 hours of 48 hour time-course where indicated. .. NIH 3T3 fibroblasts were grown in DMEM with 10% bovine calf serum (Hyclone, Logan, UT, USA).



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    rage function blocking antibody  (R&D Systems)
    94
    R&D Systems rage function blocking antibody
    Mesangial cells were grown for 2 days on tissue culture plastic + AGE-BSA or BSA (20 µg/mL) as in Figure 5 legend. 10 µg/mL rat plasma FN and 15 µg/ml <t>RAGE</t> <t>function-blocking</t> antibody were added for the final 24 hours. (A) Phase and fluorescence images were captured as described in Figure 5 legend. Average FN fluorescence intensity was decreased 1.16-fold to 0.86 ± range (n = 2) in the presence of RAGE function-blocking antibody with AGE-BSA compared to AGE-BSA treatment alone. Scale bar is 200 µm. (B) The DOC-insoluble fraction was separated by SDS-PAGE and immunoblotted with IC3 anti-rat FN monoclonal. Relative densitometry values are the mean of two experiments ± range normalized to GAPDH from the DOC soluble fraction. Blot is representative of two independent experiments.
    Rage Function Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rage+function+blocking+antibody/Human%2FMouse%2FRat+RAGE+Antibody/pmc05237628-374-2-7
    Average 94 stars, based on 1 article reviews
    rage function blocking antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Mesangial cells were grown for 2 days on tissue culture plastic + AGE-BSA or BSA (20 µg/mL) as in Figure 5 legend. 10 µg/mL rat plasma FN and 15 µg/ml RAGE function-blocking antibody were added for the final 24 hours. (A) Phase and fluorescence images were captured as described in Figure 5 legend. Average FN fluorescence intensity was decreased 1.16-fold to 0.86 ± range (n = 2) in the presence of RAGE function-blocking antibody with AGE-BSA compared to AGE-BSA treatment alone. Scale bar is 200 µm. (B) The DOC-insoluble fraction was separated by SDS-PAGE and immunoblotted with IC3 anti-rat FN monoclonal. Relative densitometry values are the mean of two experiments ± range normalized to GAPDH from the DOC soluble fraction. Blot is representative of two independent experiments.

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    Article Title: Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly

    doi: 10.1016/j.matbio.2016.07.003

    Figure Lengend Snippet: Mesangial cells were grown for 2 days on tissue culture plastic + AGE-BSA or BSA (20 µg/mL) as in Figure 5 legend. 10 µg/mL rat plasma FN and 15 µg/ml RAGE function-blocking antibody were added for the final 24 hours. (A) Phase and fluorescence images were captured as described in Figure 5 legend. Average FN fluorescence intensity was decreased 1.16-fold to 0.86 ± range (n = 2) in the presence of RAGE function-blocking antibody with AGE-BSA compared to AGE-BSA treatment alone. Scale bar is 200 µm. (B) The DOC-insoluble fraction was separated by SDS-PAGE and immunoblotted with IC3 anti-rat FN monoclonal. Relative densitometry values are the mean of two experiments ± range normalized to GAPDH from the DOC soluble fraction. Blot is representative of two independent experiments.

    Article Snippet: 15 μg/ml RAGE function blocking antibody (AF1179, R&D Systems, Minneapolis, MN) or lysyl oxidase (LOX) antibody (ab31238, Abcam, Cambridge, MA) was added for final 24 hours of 48 hour time-course where indicated.

    Techniques: Clinical Proteomics, Blocking Assay, Fluorescence, SDS Page